The study of fourteen tree wormwood genotypes grown in Tunisia highlighted different patterns of volatile metabolites concentration with mainly quantitative variability. Essential oil contents showed high amounts for Tunisian germplasm of Artemisia arborescens L. Based on clustering and multivariate analysis the observed variations pattern may be linked mainly to genetic factors. Based on this study, it is confirmed that Artemisia arborescens L. is a producer of the three commercially important compounds chamazulene, camphor and ?-thujone with various industrial purposes. The main volatile constituents identified were with considerable amounts allowing their industrial exploitation. All these findings make of Artemisia arborescens an alternative bioresource for the production of the cited valuable main compounds to replace synthetic chemical used extensively in nature which cause significant damage to environment and expose negatively on human health, fauna and flora.